Functional metagenomics

High-throughput screening

Investigation of unknown bacterial for the discovery of new bioactives compounds

This innovative approach is based on a set of high throughput screening technologies enabling the analysis of the entire genome of all microorganisms belonging to an ecological niche, including those which are not cultivable.

Hervé Blottière - Scientific director of Metafun

An automated process ensuring high throughput screening

  • 575,000

    manufactured clones

  • 23 MILLION

    cloned unknown genes

  • 400,000

    genes tested / week

  • A certified method for libraries production

    Construction of large insert genomic and metagenomic libraries

    Functional screening of large clone libraries with thousands genomic or metagenomic DNA fragments >> Identification of bacteria – cell cross-talk

    Implementation of cell-based bioassays using a high-throughput strategy >> Identification of functional bacterial genes and metabolic pathways involved in the dialogue with the host

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  • Screened signaling pathways

    Building of metagenomic libraries

    Replication of libraries

    Automatic culture of bacterial clones, followed by lysis and filtration

    Development of new screening tools (cell based assay using the reporter gene technology)

    High-throughput screening on human epithelial cells, including measurement of reporter gene activities (luminescence (HTS), fluorescence (HCS), absorbance)

    Flow cytometry analysis and cell sorting

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  • Partnerships

    Our team is ready to collaborate with the scientific and industrial communities, by applying its skills and know-how for:

    The design of functional metagenomic projects:
    – Expert advice to design the study
    – Construction of large insert genomic and metagenomic libraries
    – Functional screening and analysis

    High throughput screening from existing libraries: from the construction of the project to the analysis of the results

     Main research areas with our industrial partners:
    – Screening for microbial species/strain that may interact with the host
    – Identification of the function of a species/strain on a specific metabolic pathway: immunity, intestinal barrier, inflammation, taste receptor, oxidative stress.
    – Impact of a molecule or nutrient on microbial metabolism (synthesis or catabolism: bacterial metabolites)


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Concrete applications

Explore mechanisms/interactions between gut bacteria - human cells and discover new drugs

575,000 clones ready to be tested (with unknown bacterial genes). 18 libraries from healthy, obese, Crohn’s, CRC, Ileum/ mucosal

Identification of genes of interest for your product (probiotics, commensal bacteria…) on targeted intestinal functions (endocrine, metabolism, gut barrier, immunity)

Identification of molecules (metabolites, proteins…) of interest for drug discovery or nutrition optimization

Our reference publications in Functional metagenomics

All Publications

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