MGP SOP 001 V1

STANDARD OPERATING PROTOCOL FOR FECAL SAMPLES DNA EXTRACTION - Protocol Q

This series of MGP SOPs is the continuation of standards developments made as part of the original International Human Microbiome Standards project (IHMS). This SOP is an upgrade of IHMS_SOP 006. If you wish to consult the original IHMS SOPs, please see http://www.microbiome-standards.org/

This SOP is recommended as a replacement for IHMS_SOP 006 V2 as QIAGEN no longer makes QIAamp DNA stool kit commercially available. It requires the QIASymphony DSP Virus/Pathogen Midi Kit and the QIASymphony SP instrument from the same supplier.
This SOP is of first interest for automated, quick and reliable fecal samples DNA extraction from high biomass-containing samples, in order to characterize the fecal microbiota by metagenomic profiling. For low biomass-containing samples, or if automation is not wanted, please see MGP_SOP 002 V1 instead.

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